Pure signals
Thanks to our careful primer design, electrophoretic traces tend to maximum signal clarity in the majority of settings. If needed, primer design and PCR may be repeated until a clearly readable signal is obtained.
Full sequence or fragment length
Our Sanger applications are available for either full sequence reading or fragment length analysis.
Pseudogenes and duplicated regions
Either with the set-up by our team of experts or in cooperation with partner labs, our Sanger is suitable for the isolation/confirmation of variants even in pseudogenes or duplicated regions.
Other complex sequences
Attempts to sequence DNA stretches with particularly difficult conformations may also be done by a combination of PCR with highly specific primers and/or long-range PCR.
Custom research projects
Our Sanger is at hand for customized research projects as well, starting from samples in single tubes or in 96-well plates. Sequences may be requested in forward or forward + reverse, at different levels of deliverables: raw data, Bioinformatics, report with variant identification.
DNA extraction
DNA extraction and purification from any kind of tissue is available to initiate our Sanger sequencing services.
Contact us to take advantage of our Sanger services today:
- SANGER CARRIER: Sanger sequencing of known mutations in familial segregation studies.
- SANGER GENE: full gene sequencing of small genes by Sanger
- SANGER RESEARCH: custom sequencing projects starting from single tubes or 96-well plates.